Common projection areas of antennal and visual pathways in the honeybee brain, apis mellifera

Abstract

The convergence of primary sensory neurons of the antennae, higher order visual interneurons, and antennal motoneurons was analysed with neuroanatomical techniques in the honeybee, Apis mellifera. The different modalities evoke specific antennal responses in this insect. Three different fluorescent dyes were applied successively in the same preparation in order to visualise the various fiber projections from the antennae and the lobula in the brain of the honeybee. Three neuropile areas where sensory fibers of the antennae overlap with visual projection neurons from the lobula were found. Within the posterior-median protocerebrum the antennal tract T6-1 comes in close vicinity to the lobula tract LoT-9 and to some other lobula fibers that cannot be assigned to a special tract. Antennal T6-3 fibers overlap with lobula LoT-7 neurons within the posterior protocerebrum more laterally. Antennal T5 fibers arborise in the dorsal lobe and show common projection sites with lobula LoT-3 neurons. The multimodal convergence in the three common neuropiles demonstrates that these areas are important centers for multimodal information processing between sensory, motor, and descending neurons in insects.