Abstract
Predicting cardiovascular events remains challenging despite the range of known biomarkers. To establish relationships between various biochemical and functional parameters of the cardiovascular system. The relationship between cardiovascular dys/function and various biomarkers was examined in 145 experimental rats half of which received isoprenaline 100 mg/kg s.c. to induce cardiac impairment. Serum concentration of cardiac troponin T (cTnT), a known marker of cardiac derangement, correlated strongly with degree of myocardial injury (e.g. calcium overload, stroke volume) but correlations between cTnT and oxidative stress parameters were weak (for glutathione and vitamin C) or not found (for serum vitamin E and plasma thiobarbituric acid reactive substances levels). Relationships between cTnT and other parameters were exponential with the exception of myocardial calcium, where a power function was found. Commonly used biomarkers of oxidative stress cannot reliably predict cardiovascular dys/function in experimental rats.
Highlights
Cardiovascular diseases are a leading cause of mortality and morbidity worldwide, accounting for around 17 million of deaths each year
Cardiac troponin T, vitamin E, and vitamin C were measured in serum, thiobarbituric acid reactive substances (TBARS) in plasma, antioxidant enzymes SOD and GPx in erythrocytes and the total glutathione in the whole blood
Standard approaches were used for the analysis: SOD and GPx were determined by commercial kits (Randox, United Kingdom), cardiac troponin T (cTnT) by electroluminescence immunoassay (Roche Diagnostics, Germany), vitamin E by fluorimetric detection, the total blood glutathione and vitamin C by UV detection
Summary
Predicting cardiovascular events remains challenging despite the range of known biomarkers. Aim. To establish relationships between various biochemical and functional parameters of the cardiovascular system. The relationship between cardiovascular dys/function and various biomarkers was examined in 145 experimental rats half of which received isoprenaline 100 mg/kg s.c. to induce cardiac impairment. Serum concentration of cardiac troponin T (cTnT), a known marker of cardiac derangement, correlated strongly with degree of myocardial injury (e.g. calcium overload, stroke volume) but correlations between cTnT and oxidative stress parameters were weak (for glutathione and vitamin C) or not found (for serum vitamin E and plasma thiobarbituric acid reactive substances levels). Relationships between cTnT and other parameters were exponential with the exception of myocardial calcium, where a power function was found. Used biomarkers of oxidative stress cannot reliably predict cardiovascular dys/function in experimental rats.
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