Commom, well-documented and rare HLA alleles observed in the brazilian bone marrow registry

Abstract

Aim We intend to characterize alleles with 5 or more counts in non related donors of the Brazilian Bone Marrow Registry (REDOME) that are not listed in common and well documented (CWD) alleles version 2. Methods Identification and comparison of alleles and NMDP alleles codes which first allele in the subtype are not in CWD version 2 from the REDOME data base at February 15, 2015. Results We identified 30,395 HLA typings with 1575 alleles or NMDP alelle codes of not common or not well documented among the 3,227,387 donors. A total of 1213 donor registries corresponding to 158 alleles in A locus, 306 in B locus, 47 in C locus, 136 in DRB1 locus and 37 in DQB1 locus had less of 5 counts. In a few cases (28/283) it was possible to have access to the outputs and from these, 11 were probably misinterpreted results, although in one case A∗02:90 was also reported in Chinese donor registry. We also identified 259 alleles with 5–25 counts, 116 alleles with 26–100 counts, 29 alleles with 101–500, and 4 with more than 500. Eighty of these alleles or NMDP allele were most likely misinterpretation due to SSO bead adjustments corresponding to 9582 donors. Actual SSO output reviewed interpretation and SBT confirmed typings are show in Table 1. Conclusion Sequencing based typings were on going to confirm these Brazilian CWD HLA alleles and are displayed at www.imunogenetica.org (in Portuguese). Download : Download full-size image