Committed for the long haul: Do nonapeptides regulate long-term pair maintenance in zebra finches?

Abstract

The nonapeptides (oxytocin, vasopressin, and their non-mammalian homologs) regulate a number of social behaviors across vertebrates including monogamous pair bonds in mammals. Recent work on zebra finches has shown an important role for these neurohormones in establishing avian pair bonds as well. However, studies on the role of nonapeptides in maintaining pair bonds after pair formation are lacking. The goal of the present study was to investigate the effects of an oxytocin receptor antagonist (OTA) on pair maintenance behaviors in the monogamous zebra finch. I injected established zebra finch pairs over three days with either 5 μg of an OTA or a vehicle control, and separated the partners for one hour, after which partners were reunited and their reunion recorded on video for 30 min. Videos were then coded to measure singing, affiliative (allopreening, clumping, following), and aggressive (pecking) behaviors. These behaviors were also measured both on the day before injections to establish a pre-treatment level and two days after the last injection. Control and antagonist treated birds did not differ in the amount of time spent clumping or the frequency of pecking across the experiment. However, both male and female zebra finches that received OTA significantly reduced the amount of time spent following their partner. Females given the OTA treatment reduced allopreening and males given the OTA treatment reduced the frequency of singing bouts directed at their partners relative to controls. These results suggest that the nonapeptides play a role in regulating some, but not all, pair maintenance behaviors in experienced zebra finches.