Abstract
Sera from patients with hepatitis non-A, non-B as well as sera from blood donors were tested for HCV antibodies using eight enzyme-linked immunosorbent assay (ELISA) kits from different producers (Abbott, Ortho, Wellcome, Sanofi Pasteur, Organon, Gen. Biol., Innogenetics, Sorin) and two immunoblots (RIBA II and LIA). Most ELISA kits were well suited for the diagnosis of HCV infection in hepatitis patients, especially when results were corroborated by an immunoblot. However, results from sera from blood donors are much more difficult to interpret. Out of 63 sera tested only 23 gave concordant positive results in all eight ELISAs and only nine more sera were eventually judged positive. This meant that in 31 cases the final result was inconclusive or a false positive. Immunoblots were of limited value for corroboration of sera with discordant ELISA results. A high number of discordant results ( 13 30 , 43%) between the two immunoblots were found in those sera. For HCV testing of blood donors the diagnostic kits need to be improved.
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