Abstract
Commentary on the preservation of human RBC, platelets, plasma clotting proteins and peripheral blood mononuclear hematopoietic stem cells by freezing and storage in a 80 C mechanical freezer
Highlights
NBRL has demonstrated that RBC preserved with 40% W/V glycerol, platelets preserved with 6% DMSO, peripheral blood pluripotential hematopoietic mononuclear adult stem cells preserved with 10% DMSO and plasma can all be frozen and stored in mechanical freezers at -80°C
The 50 year experience at the NBRL, Boston, MA has demonstrated that frozen red blood cell cells, frozen platelets, and frozen plasma can be used to successfully treat wounded casualties without any adverse events
Human RBC have been successfully frozen with 40% W/V glycerol at -80°C for at least 21 years [4]; human platelets have been successfully frozen at -80°C with 6% DMSO for at least 2 years [5,6] human plasma has been successfully frozen at -80°C for at least 14 years [7] and human peripheral blood mononuclear hematopoietic stem cells have been successfully frozen at -80°C for at least 11⁄2 years [8]
Summary
NBRL has demonstrated that RBC preserved with 40% W/V glycerol, platelets preserved with 6% DMSO, peripheral blood pluripotential hematopoietic mononuclear adult stem cells preserved with 10% DMSO and plasma can all be frozen and stored in mechanical freezers at -80°C.
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