Abstract
Commentary: Muscle Microbiopsy to Delineate Stem Cell Involvement in Young Patients: A Novel Approach for Children With Cerebral Palsy.
Highlights
We appreciate the opportunity to comment on the above-referenced article from our colleagues in Belgium
We showed that myoblast progenitors derived from the same satellite cell (SC) had decreased capacity to fuse and to differentiate into myotubes in vitro (Domenighetti et al, 2018)
Without preplating (Yoshioka et al, 2020), growth of primary myogenic cultures over time will result in non-myogenic enriched populations of cells (Rando and Blau, 1994), while SCs will differentiate into myoblasts and rapidly lose their potential to self-renew and contribute to muscle fiber formation (Cosgrove et al, 2009)
Summary
We appreciate the opportunity to comment on the above-referenced article from our colleagues in Belgium. We previously performed a number of these studies (Lieber et al, 2003; Smith et al, 2009, 2011, 2012, 2013; Dayanidhi et al, 2015; Domenighetti et al, 2018), including three relevant investigations on resident muscle stem cell homeostasis in contractured CP muscle (Smith et al, 2013; Dayanidhi et al, 2015; Domenighetti et al, 2018). Their study lacked clarity regarding the actual muscle stem cell population being studied. This is based on the method chosen to extract myogenic cells from the very small biopsy and the need to expand and passage these cells multiple times before FACS analysis. Without further characterization of these cells, it is very difficult to interpret their results in light of muscle stem cell biology
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