Abstract
Live imaging and single cell tracking enables researchers to monitor crucial aspects of the biology of neural populations. In this commentary, we highlight the requirements, applications, and limitations of a protocol recently published by our research group. This protocol involves adapting the culture of several types of neural cells to time-lapse video microscopy, and the post-processing of the data to track distinct cell populations.
Highlights
The correct hardware and software settings constitute only part of a successful live imaging experiment
We recently presented a feasible protocol that enables researchers to perform single cell tracking by time-lapse video-microscopy, and which can be followed by data post-processing[22]
We describe the use of dedicated software for single cell tracking that was developed by the group of Dr Timm Schroeder, namely The Tracking Tool[23]
Summary
The correct hardware and software settings constitute only part of a successful live imaging experiment. Despite constituting one of the oldest scientific approaches[7], live imaging and single cell tracking provides real-time information over the length of an experiment, thereby allowing variations in the events listed above to be described precisely5, . Commentary: Live Imaging Followed by Single Cell Tracking to Monitor the Cell Biology and Lineage Progression of Multiple Neural Populations.
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