Abstract
We read with interest the article entitled “Validation and implementation of a direct RT-qPCR method for rapid screening of SARS-CoV-2 infection by using non-invasive saliva samples” (Brotons et al., 2021). This study validates and implements an optimized screening method for the detection of SARS-CoV-2 ribonucleic acid, integrating the use of self-collected raw saliva samples, single-step heat-treated virus inactivation and ribonucleic acid extraction, and direct reverse transcription-quantitative polymerase chain reaction (RT-qPCR).
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