Comment on: Comparison of three lateral flow immunochromatographic assays for the rapid detection of KPC, NDM, IMP, VIM and OXA-48 carbapenemases in Enterobacterales.

Abstract

We recently published a manuscript entitled ‘Comparison of three lateral flow immunochromatographic assays for the rapid detection of KPC, NDM, IMP, VIM and OXA-48 carbapenemases in Enterobacterales’ by Bernabeu et al.1 This correspondence aimed to compare the performances of three lateral flow immunoassays (LFIAs) for the detection of carbapenemases. On a collection of 199 Enterobacterales, performance of Coris K.O.N.V.I. RESIST-5 (Coris BioConcept, Gembloux, Belgium) was established as 94.9% and 93.3% for sensitivity and specificity, respectively. The lower specificity compared with that of NG-Test CARBA-5 (NG Biotech, Guipry-Messac, France), 98.1%, was mainly due to the detection of MBLs. It included bad performance for the detection of IMP enzymes, as well as low intensity bands observed for NDM-, IMP- and VIM-type carbapenemases. However, during the publication process of our manuscript, Coris BioConcept adapted its protocol to improve the performances and the usability of the RESIST-5 K.O.N.V.I. assay. Modifications of the protocol included simplification of the procedures. In the previous version, two different buffers were necessary whereas only one is used in the new version. Additionally, for the former procedure a pipette was needed to distribute one of the two buffers whereas the new test contains a dropper for the unique lysis buffer.