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Abstract
We address the criticism of our methodology for determination of the three-body cooperative terms in the potential of mean force (PMF) of the hydrophobic interaction of methane molecules in water [Czaplewski et al., Prot. Sci. 9, 1235 (2000)] expressed in the title paper of Shimizu and Chan, as well as their conclusion that hydrophobic association is predominantly anti-cooperative. We demonstrate that their reference two-methane PMF curve is subject to a systematic error, which invalidates their conclusions about the sign of the cooperative PMF.
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