Comet assay on mice testicular cells

Abstract

Event Abstract Back to Event Comet assay on mice testicular cells Anoop K. Sharma1* 1 Technical University of Denmark, Division of Toxicology & Risk Assessment, Denmark Heritable mutations may result in a variety of adverse outcomes including genetic disease in the offspring. In recent years the focus on germ cell mutagenicity has increased and the “Globally Harmonized System of Classification and Labelling of Chemicals (GHS)” has published classification criteria for germ cell mutagens (Speit et al., 2009). The in vivo Comet assay is considered a useful tool for investigating germ cell genotoxicity. In the present study DNA strand breaks in testicular cells of mice were investigated. Different classes of chemicals were tested in order to evaluate the sensitivity of the comet assay in testicular cells. The chemicals included environmentally relevant substances such as Bisphenol A, PFOS and Tetrabrombisphenol A. Statistical power calculations will be presented to aid in the design of future Comet assay studies on testicular cells. Power curves were provided with different fold changes in % tail DNA, different number of cells scored and different number of gels (Hansen et al., 2014). An example is shown in Figure 1. A high throughput version of the Comet assay was used. Samples were scored with a fully automatic comet assay scoring system that provided faster scoring of randomly selected cells. Figure 1 Acknowledgements Acknowledgements Merethe Kjær Hansen1, Murat Kulachia1 and 3, Marianne Dybdahl2, Julie Boberg2 and Vivian Jørgensen2. 1 Technical University of Denmark, Dept. of Applied Mathematics and Computer Science. 2 Technical University of Denmark, National Food Institute. 3 Luleå University of Technology, Dept.of Business Administration, Technology and Social Sciences. References Speit, G., Vasquez, M., Hartmann, A. The comet assay as an indicator test for germ cell genotoxicity. Mutation Research/Reviews in Mutation Research. 2009 (681) 3-12. Hansen, M.K., Sharma, A.K., Dybdahl, M., Boberg, J., Kulahci, M. In vivo Comet assay - statistical analysis and power calculations of mice testicular cells. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2014. (774) 29-40. Keywords: in vivo, Testis, High-Throughput Screening Assays, DNA strand breaks, Power calculation, Statistical issues Conference: ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015. Presentation Type: Oral Presentation Topic: Statistical issues within comet assay studies Citation: Sharma AK (2015). Comet assay on mice testicular cells. Front. Genet. Conference Abstract: ICAW 2015 - 11th International Comet Assay Workshop. doi: 10.3389/conf.fgene.2015.01.00025 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 02 Jun 2015; Published Online: 23 Jun 2015. * Correspondence: PhD. Anoop K Sharma, Technical University of Denmark, Division of Toxicology & Risk Assessment, Søborg, 2860, Denmark, aksha@food.dtu.dk Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Anoop K Sharma Google Anoop K Sharma Google Scholar Anoop K Sharma PubMed Anoop K Sharma Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.