Abstract
Publisher Summary Single-cell gel electrophoresis (SCGE, comet assay) provides a very sensitive method for detecting strand breaks and measuring repair kinetics at the level of single cells. The SCGE assay is a particularly valuable technique because it allows the detection of intercellular differences in DNA damage and repair in any eukaryotic cell population. A variety of possible modifications of the assay facilitates the detection of single-stranded DNA breaks and alkali labile sites, double-stranded DNA breaks, incomplete excision repair sites, and interstrand cross-links, and increases the specificity and sensitivity of the assay. Moreover, it enables the study of different DNA repair pathways such as base excision and nucleotide excision repair. In addition, DNA fragmentation associated with cell death or related to apoptosis can be evaluated with the comet assay. There is also a great variety of DNA damaging agents that can be used to study DNA damage and repair with the comet assay procedure.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
