Combining UFLC-QTOF-MS analysis with biological evaluation of Centrosema coriaceum (Fabaceae) leaves.

DANIELLE M.O. ARAGÃO,LARA M. CAMPOS,RODRIGO L. FABRI,ANA CAROLINA M. APOLÔNIO,JULIANA T. GRANATO,THALITA F. SOUZA,ERICK E. OLIVEIRA,ANA PAULA FERREIRA,ARI S.O. LEMOS

doi:10.1590/0001-3765202220200491

DANIELLE M.O. ARAGÃO, LARA M. CAMPOS + Show 7 more

Open Access

https://doi.org/10.1590/0001-3765202220200491

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Abstract

Centrosema coriaceum Benth belongs to Fabaceae family and have few studies of biological activity and chemical composition. Thus, the aims of this work were to determine chemical profile of the ethanolic extract of C. coriaceum leaves (CCE) by UFLC-QTOF-MS and to evaluate its in vitro biological potential. CCE showed MIC value of 1000 µg/mL against Candida glabrata (fungistatic effect) and high affinity in cell envelope by increasing cell permeability in nucleotide leakage, sorbitol and ergosterol assays. CCE showed antioxidant activity in all assays performed. For the anti-inflammatory and cytotoxicity activities, CCE, at all tested concentrations, significantly inhibited the production of nitric oxide and did not decrease J774A.1 cell viability below 70%. Finally, rutin, kaempferol-3O-rutinoside, caffeic acid, and sucrose were identified in CCE by UFLC-QTOF-MS. These results suggest, for the first time, that C. coriaceum has interesting antifungal, antioxidant, and anti-inflammatory activities.

Highlights

IntroductionFungal diseases are responsible for much of morbidity and mortality in public health systems (Parente-Rocha et al 2017), causing at least 1.4 million human deaths per year worldwide (Sanglard 2016)
Centrosema coriaceum Benth belongs to Fabaceae family and have few studies of biological activity and chemical composition
Our research group conducted this work, with C. coriaceum ethanolic extract looking for its biological activities and studying its chemical composition, hoping that it could be a new source of bioactive molecules in the treatment of fungal diseases, oxidative stresses and inflammatory process

Summary

Introduction

Fungal diseases are responsible for much of morbidity and mortality in public health systems (Parente-Rocha et al 2017), causing at least 1.4 million human deaths per year worldwide (Sanglard 2016). The presence of pathogenic Candida spp. fungi in the body may lead to candidiasis conditions that are accompanied by inflammatory processes (Roselletti et al 2017). This process releases chemical mediators in the affected tissue, including excitatory amino acids, oxygenreactive species (ROS) and other free radicals, peptides, lipids and cytokines (Chen et al 2016). The presence of resistance genes (Candida Drug Resistance - CDR and Multidrug Resistance - MDR) in yeasts of the genus Candida (Khosravi et al 2016) associated with side effects, toxicity, and inefficiency from current candidiasis treatment, have become challenging antifungal therapy through available drugs (Bhattacharjee 2016, Perlin et al 2017).

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