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Abstract
Aminopropyl-functionalized SBA-15 mesoporous silica was used as a support to adsorb myoglobin. Then, in order to avoid the leakage of adsorbed myoglobin, lysozyme was covalently tethered to the internal and external surface of the mesoporous silica with glutaraldehyde as the coupling agent. The property of amino-functionalized mesoporous silica was characterized by N2 adsorption-desorption and thermogravimetric (TG) analysis. The feature of the silica-based matrix before and after myoglobin adsorption was identified by fourier transform infrared (FTIR) and UV/VIS measurement. With o-dianisidine and H2O2 as the substrate, the peroxidase activity of adsorbed myoglobin was determined. With Micrococus lysodeilicus as the substrate, the antibacterial activity of covalently tethered lysozyme was measured. Results demonstrated that the final product not only presented peroxidase activity of the myoglobin but yielded antibacterial activity of the lysozyme.
Highlights
In recent years, the enzyme has been employed in numerous applications in the form of immobilized enzyme preparation [1−3]
The enzyme molecular is sensitive to adverse effects of some reagents in the solution, which may cause the loss of the activity of the enzyme. (II) In contrast, for the enzyme which is embedded in the support and finitely contacts with the solution, the catalytic activity of the enzyme in the support is only observed if the diffusion of the product and the substrate in the support is similar to the case in the solution [4]
The aminopropyl-functional SBA-15 mesoporous material was first synthesized by one-pot co-condensation synthesis in 1996 [14]
Summary
The enzyme has been employed in numerous applications in the form of immobilized enzyme preparation [1−3]. In the sol-gel method, the support presents poor mechanical stability and the pore size distribution cannot be controlled These disadvantages block practical application of immobilized enzyme in industry. When the spatial size of the enzyme molecule is smaller than the pore size, many kinds of mesoporous molecular sieves are used as support to achieve immobilization through physical adsorption. Due to their high mechanical stability, good chemical stability, large special surface area and pore volume, a silica-based matrix such as MCM-41 [11], SBA-15 [12] is widely applied to the adsorption of enzyme.
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