Abstract
Nerve tissue engineering is an important strategy for the treatment of brain injuries. Mesenchymal stem cell (MSC) transplantation has been proven to be able to promote repair and functional recovery of brain damage, and poly (lactic-co-glycolic acid) (PLGA) has also been found to have the capability of bearing cells. In the present study, to observe the ability of PLGA scaffold in supporting the adherent growth of MSCs and neurons in vivo and vitro and to assess the effects of PLGA scaffold on proliferation and neural differentiation of MSCs, this study undertakes the following steps. First, MSCs and neurons were cultured and labeled with green fluorescent protein (GFP) or otherwise identified and the PLGA scaffold was synthesized. Next, MSCs and neurons were inoculated on PLGA scaffolds and their adhesion rates were investigated and the proliferation of MSCs was evaluated by using MTT assay. After MSCs were induced by a neural induction medium, the morphological change and neural differentiation of MSCs were detected using scanning electron microscopy (SEM) and immunocytochemistry, respectively. Finally, cell migration and adhesion in the PLGA scaffold in vivo were examined by immunohistochemistry, nuclear staining, and SEM. The experimental results demonstrated that PLGA did not interfere with the proliferation and neural differentiation of MSCs and that MSCs and neuron could grow and migrate in PLGA scaffold. These data suggest that the MSC-PLGA complex may be used as tissue engineering material for brain injuries.
Highlights
In recent years, the development of tissue engineering has provided a new strategy for the repair of tissue injuries
The aim of this study is to evaluate whether the Mesenchymal stem cell (MSC)-PLGA scaffold complex is a potential tool for the treatment of brain injuries
Merge (b) MSCs absorbance in the PLGA and control groups after incubation for 7 days (P < 0 05) (Figure 3(c)). These results suggest that the PLGA scaffold did not interfere with the proliferation and neuronal differentiation of MSCs in vitro
Summary
The development of tissue engineering has provided a new strategy for the repair of tissue injuries. The core of tissue engineering is to construct new tissue substitutes composed of biological materials and cells for promoting the recovery and maintenance of biological functions [1, 2]. (lactic-co-glycolic acid) (PLGA) is one biodegradable polymer material and the degradation time of PLGA can be adjusted by altering the ratio of lactic acid and glycolic acid in its copolymer for particular applications. PLGA with a ratio of 75 : 25 of PLA : PGA showed great stability in body fluids (pH 7.2) with an optimum degradation rate (9% to 12% or so), and axons could regenerate into the implanted PLGA scaffolds in rats subjected to thoracic spinal cord transection injury [4]. Mesenchymal stem cells (MSCs) could differentiate into neuron-like cells under
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