Abstract
Automated ultra-performance liquid chromatography (LC) with a radiometric detection system has been developed to analyze compounds labeled with short-lived positron (β +)-emitting radionuclides in microdialysates. This system involves (1) on-line microdialysis sampling to submit dialysates directly into the LC, (2) ultra-performance LC for improving sensitivity, resolution and speed of analysis, and (3) tandem flow-through of β + and electrochemical detectors for real-time monitoring of radioactive and endogenous compounds simultaneously. This system permitted ultrasensitive measurement of radioactive compounds at the lowest detection limit of about 1 Bq with a high-temporal resolution of sampling and less operator attention. The developed system was used for the continuous monitoring of l-[β- 11C]-3,4-dihydroxyphenylalanine ( l-[β- 11C]DOPA) metabolites in the rat brain.
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