Abstract
Infectious diseases are considered the greatest threat to the modern high-density shrimp aquaculture industry. Specificity, rapidity, and sensitivity of molecular diagnostic methods for the detection of asymptomatic infected shrimp allows preventive measures to be taken before disease outbreaks. Routine molecular detection of pathogens in infected shrimp can be made easier with the use of a direct polymerase chain reaction (PCR). In this study, four direct PCR reagent brands were tested, and results showed that the detection signal of direct PCR in hepatopancreatic tissue was more severely affected. In addition, portable capillary electrophoresis was applied to improve sensitivity and specificity, resulting in a pathogen detection limit of 25 copies/PCR-reaction. Juvenile shrimp from five different aquaculture ponds were tested for white spot syndrome virus infection, and the results were consistent with the Organization for Animal Health’s certified standard method. Furthermore, this methodology could be used to examine single post larvae shrimp. The overall detection time was reduced by more than 58.2%. Therefore, the combination of direct PCR and capillary electrophoresis for on-site examination is valuable and has potential as a suitable tool for diagnostic, epidemiological, and pathological studies of shrimp aquaculture.
Highlights
As a globally traded animal providing agri-food with high quality protein, the global market for shrimp aquaculture was valued at USD 28 billion in 2018 [1,2]
Major shrimp infectious disease outbreaks on a global scale occur every few years, including white spot disease (WSD), acute hepatopancreatic necrosis disease (AHPND), and hepatopancreatic microsporidiosis (HPM), which have become prevalent in recent years [4]
The specific nucleic acid primers used in this study were referenced from previous reports [29,30,31], and detailed information is listed in Table 1, including the VP28 envelope protein gene of white spot syndrome virus (WSSV), the Photorhabdus insect-related (Pir) toxin gene in a plasmid of Vibrio parahaemolyticus, and the spore wall protein gene of Enterocytozoon hepatopenaei
Summary
As a globally traded animal providing agri-food with high quality protein, the global market for shrimp aquaculture was valued at USD 28 billion in 2018 [1,2]. The. World Organization for Animal Health (OIE) has standardized assays for major shrimpborne diseases, most of which use nested PCR or real-time PCR technology [14]. World Organization for Animal Health (OIE) has standardized assays for major shrimpborne diseases, most of which use nested PCR or real-time PCR technology [14] These standard methods have high sensitivity and specificity for pathogen detection, the operational procedures are complicated, so they must be performed in a well-equipped laboratory. As a result, both the time and money needed for detection remain high, which limits the application of routine molecular detection and on-site examination of infectious diseases in aquaculture. After comparison with an OIE-certified system for disease detection of white shrimp in aquaculture ponds from different regions, we verified that this technology platform (i.e., direct PCR-based CE) is suitable for on-site disease surveillance in aquaculture
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
