Combining ability of soybean (Glycine max L.) yield performance and related traits under water-limited stress conditions

Abstract

Development of improved genotypes requires acquiring adequate information on genetic background and nature of gene action for planning appropriate breeding and selection strategies. This study was aimed at obtaining estimates of general and specific combining ability, identifying the best combiners in developing drought tolerant genotypes and determining gene action of soybean traits. Six genotypes were crossed using the North Carolina II mating design to form eight families, of which Makwacha and Nasoko were regarded as males and TGX1990-137F, TGX1987-23F, 1988-70F and Tikolore were regarded as females. Two-hundred F2 progeny and six parents were evaluated in the field under non-water-limited stress (non-WLS) and water-limited stress (WLS) conditions across 2 sites during the 2018 dry season in a split-plot layout. Water-regimes were allocated to main plots and genotypes to subplots in a randomized complete block design with 3 replications. Significant genotype and water regime effects were found for seed yield and yield components. Most progeny ranked higher than parents under WLS conditions for all traits. Significant general combining ability (GCA) and specific combining ability (SCA) effects for all traits indicated that both additive and non-additive gene effects were important for trait expression under WLS conditions. Both GCA and SCA effects significantly interacted with water regime effects, demonstrating the need for testing of potential progeny across varied WLS levels to determine stable genotypes. Parents and F2 progeny with significant positive GCA and SCA effects, respectively, under WLS were identified as potential material for inclusion in the breeding program for drought tolerance.