Combining ability analysis for Phaeosphaeria leaf spot resistance and grain yield in tropical advanced maize inbred lines

Abstract

Phaeosphaeria leaf spot (PLS) disease (causal agent Phaeosphaeria maydis (Henn.) Rane, Payak & Renfro) of maize is increasing in importance in sub-Saharan Africa (SSA). However, there is still limited information on the combining ability for disease resistance of the germplasm that are adapted to African environments. Evaluating combining ability effects and their interactions with the environment would provide valuable information that can be used in the development of cultivars that are resistant to PLS. This study was therefore conducted to determine the combining ability, gene action and the relationship between grain yield and PLS disease severity among selected tropical advanced maize inbred lines. Forty five F 1 hybrids were generated by crossing 10 inbred lines in a half diallel mating scheme. The 45 hybrids along with the ten inbred parents were evaluated in four environments, with two replications each between 2007 and 2009. General and specific combining ability (GCA and SCA) effects were highly significant ( P ≤ 0.001) for PLS, grain yield and days to anthesis. GCA effects accounted for 66–90% and SCA effects for 10–34% of the variation in the hybrids for PLS resistance, grain yield and days to anthesis. This indicated predominance of additive over non-additive gene action for the three traits in these inbred lines. The resistant inbred lines to PLS were A1220-4, N3-2-3-3, CML312, MP18 and CML488. These lines had good combining ability for PLS resistance and contributed towards resistance in their crosses. In general, resistant hybrids involved a susceptible and a resistant parent, where at least one of the parents had a negative GCA effect. In addition, lines A1220-4 and CML312 contributed towards high yield and were late maturing. Inbred line CZL00009 conferred genes for early maturity. Linear regression analysis indicated that grain yield of maize was suppressed by about 250 kg ha −1 per each increase in PLS disease severity score, underscoring the need to control the disease. Significant ( P < 0.01), negative correlations ( r = −0.29 to −0.43) between grain yield and PLS severity were also detected. This showed the potential of PLS to reduce yield when favourable conditions for disease development are present. By and large, highly significant additive gene action implied that progress would be made through selection. Although non-additive effects were small (±10%), observation of dominance effects which were associated with reduced disease levels in some hybrids may be exploited in developing single cross maize hybrids among these inbreds when one parent is resistant.