Abstract
Yarrowia lipolytica lipase Lip2 (YlLip2) is an important biocatalyst for ester synthesis, biodiesel production and enantiomer resolution. The YlLip2 with an N-terminal histidine-tag (His 6-YlLip2) was successfully expressed in Pichia pastoris . Three different cultivation strategies had been compared for the production of His 6-YlLip2 by P. pastoris using a 10-l bioreactor. The results showed that His 6-YlLip2 activity and cell viability could be greatly improved by employing the combined strategies. Using a low salt medium (LSM) instead of the basal salt medium (BSM) and lowering the temperature from 28 to 25°C, the maximum His 6-YlLip2 activity and volumetric productivity were respectively increased by 55.3 and 79.8%. The production of His 6-YlLip2 and cell viability was further improved by combining sorbitol co-feeding with methanol. In this culture strategy, the maximum activity of His 6-YlLip2 reached 15,600 U/ml after 114 h of induction. The cell mortality decreased by 11.2% (while the control decreased about 27.6%) after 120 h methanol induction. The N-terminal histidine-tag brought convenience to purification. The molecular weight of His 6-YlLip2 was about 38 kDa. The pure His 6-YlLip2 presented a specific activity of 4,830 U/mg when olive oil was used as the substrate.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
