Combined proteomics and transcriptomics identifies serpin family C member 1 associated protein as a biomarker of endometriosis

Abstract

Objective To explore potential biomarkers indicating endometriosis (EM). Materials and methods A proteomics method and combined quantitative transcriptomics were adopted to highlight markers in the EM. Venn analysis was used to integrate the ribonucleic acid sequencing (RNA-seq) and protein profiles. Promising candidate markers were tested by enzyme-related immunosorbent assay. Results A sum of 979 mRNAs and 39 proteins were tested to be significantly differentially expression in the standard cluster compared with the EM cluster. Venn analysis showed a filtered signature of only two down-regulated molecules in the EM group, i.e. fetuin B (FETUB) and serpin family C member 1 (SERPINC1); the latter showed a big variance between the control category and the EM set in the authentication test. Conclusion SERPINC1 may be a useful possible biomarker for the analysis of EM.