Abstract
High-performance liquid chromatography (HPLC) has been used for the preparative separation of environmentally relevant aliphatic and polycyclic aromatic hydrocarbons (PAHs), and for quantifying PAHs. Normal-phase HPLC was employed as a fractionation step, whereas the quantitative determination of the PAHs was accomplished by using reversed-phase HPLC. In both cases, GC and GC/MS were used as confirmatory techniques. The concentrations of twelve unsubstituted PAHs in sixteen surface and deeper sediments, taken from the northern and central Adriatic Sea, are reported, together with an evaluation of their dependence upon the percentages of fine material and total organic carbon.
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