Combined pollution effects of Cu and benzotriazole in rice (Oryza sativa L.) verified by split-root experiment.

Abstract

Although the combined effect of organic ligands and heavy metals in the environment on plants have been frequently reported, their complexed interaction in plants and the physiological effects remain to be revealed. Metal complexing agent benzotriazole (BTR) has extensive environmental pollution. In this study, root-splitting experiments were designed to identify the in vivo and in vitro effects of BTR on the accumulation and translocation of Cu in rice (Oryza sativa L.), and the concentrations and translocation factor (TF) of Cu and BTR in different parts of rice were measured. In the in vitro interaction treatments, low BTR concentrations enhanced Cu uptake and lateral transport in rice, while higher levels of BTR's exposure (i.e., ≥ 100 μM) resulted in opposite effects. Differently, significant increase in the lateral transport of Cu and vertical translocation of BTR in rice presented in the in vivo interaction treatments. TF of Cu from root A to root B (TFRA-RB) increased from 0.05 to 0.272 with the BTR concentration increasing from 0 to 100 μM, and higher TF of BTR from root to shoot (TFR-S), ranging from 1.00 to 1.75, compared with single BTR exposure treatments was observed. The phytotoxicity of BTR expressed by the catalase activity was significantly alleviated by the in vivo accumulated Cu in rice.