Abstract
BackgroundThe use of Plasma Rich in Growth Factors (PRGF) and Adipose Derived Mesenchymal Stem Cells (ASCs) are today extensively studied in the field of regenerative medicine. In recent years, human and veterinary medicine prefer to avoid using traumatic techniques and choose low or non-invasive procedures. The objective of this study was to evaluate the efficacy of PRGF, ASCs and the combination of both in wound healing of full-thickness skin defects in rabbits. With this purpose, a total of 144 rabbits were used for this study. The animals were divided in three study groups of 48 rabbits each depending on the administered treatment: PRGF, ASCs, and PGRF+ASCs. Two wounds of 8 mm of diameter and separated from each other by 20 mm were created on the back of each rabbit: the first was treated with saline solution, and the second with the treatment assigned for each group. Macroscopic and microscopic evolution of wounds was assessed at 1, 2, 3, 5, 7 and 10 days post-surgery. With this aim, 8 animals from each treatment group and at each study time were euthanized to collect wounds for histopathological study.ResultsWounds treated with PRGF, ASCs and PRGF+ASCs showed significant higher wound healing and epithelialization rates, more natural aesthetic appearance, significant lower inflammatory response, significant higher collagen deposition and angiogenesis compared with control wounds. The combined treatment PRGF+ASCs showed a significant faster cutaneous wound healing process.ConclusionsThe combined treatment PRGF+ASCs showed the best results, suggesting this is the best choice to enhance wound healing and improve aesthetic results in acute wounds.
Highlights
The use of Plasma Rich in Growth Factors (PRGF) and Adipose Derived Mesenchymal Stem Cells (ASCs) are today extensively studied in the field of regenerative medicine
Wound closure and epithelialization rate evaluation Wounds treated with PRGF, ASCs or combination of both showed significant faster wound healing rates compared to control wounds throughout the study except at day 10 (p < 0.001)
When single therapies were compared, ASCs demostrated at day 7 to accelerate the wound healing process more efficiently than PRGF (p < 0,001) (Fig. 2)
Summary
The use of Plasma Rich in Growth Factors (PRGF) and Adipose Derived Mesenchymal Stem Cells (ASCs) are today extensively studied in the field of regenerative medicine. Regenerative skin wound therapy is a novel and rapidly developing field of biomedical research that aims to promote wound healing [6] and focuses on replacing, restoring and regenerating damaged cells, tissues and organs [7] Stem cells, with their properties to self-renew and undergo differentiation are being extensively assessed for their wound healing potential [8]. Chicharro et al BMC Veterinary Research (2018) 14:288 cells (ASCs) are an appealing source of mesenchymal stem cells (MSCs) due to their abundant availability, good expansion capacity, their ability to proliferate in culture, and cryopreservation capacity [9] They have been already successfully used in the treatment of soft tissue defects, scars, and burn injuries by showing an acceleration and improvement on the quality of the wound healing process [10]
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