Abstract

Multiphoton microscopy (MPM) and optical coherence tomography (OCT) are two important techniques for non-invasive and high-resolution imaging of highly scattering biomedical tissues. MPM and OCT provide complementary information about tissues as the detected signals originate from different substances and mechanisms. Combining MPM and OCT can simultaneously provide rich functionality and morphology information about biomedical tissues. We report a novel system which combines multiphoton and optical coherence microscopy. A femtosecond Ti:Sapphire laser is used as the light source which has a pulse width of 10 fs and a spectral bandwidth of 100 nm. The ultrafast Ti:Sapphire laser provides the short pulse and the broad bandwidth required by high-resolution MPM and OCT, respectively. By matching the resolution and the imaging volume of MPM and OCT, we achieved co-registration between the MPM and OCT images. Dispersion compensation is critical in this MPM/OCT system and its effect is discussed. Representative images of co-registered MPM/OCT are presented.

Highlights

  • Multiphoton microscopy (MPM)[1] and optical coherence tomography (OCT) 2 are two important techniques for noninvasive and high-resolution imaging of highly scattering biomedical tissues

  • MPM and OCT provide complementary information about tissues as the detected signals originate from different substances and mechanisms in the tissues

  • We report a novel system which combines multiphoton microscopy and optical coherence tomography

Read more

Summary

INTRODUCTION

Multiphoton microscopy (MPM)[1] and optical coherence tomography (OCT) 2 are two important techniques for noninvasive and high-resolution imaging of highly scattering biomedical tissues. It will be very useful to have a combined MPM/OCT system on the same platform where high resolution MPM and OCT images can be simultaneously taken from the same sampling area. High resolution MPM/OCT can be achieved without the need of a pinhole design In this manuscript, we report a novel system which combines multiphoton microscopy and optical coherence tomography. High-resolution MPM and OCT imaging can be achieved with the same ultrafast laser. With such a broad bandwidth, pulse broadening and dispersion compensation are critical in this MPM/OCT system, which will be discussed in details . Preliminary images of co-registered MPM/OCT will be presented

SYSTEM DESIGN
SAMPLE PREPARATION
RESULTS AND DISCUSSIONS
CONCLUSIONS

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.