Abstract
A scheme for screening of micromycetes - producers of proteases with the activity of hemostasis system proteins, based on their enzymatic indices determination and the activity towards chromogenic peptide substrates for proteins of the hemostasis system was developed. Depending on the ability of proteases producers to cleave such substrates, an enzymatic reaction in conditions containing human plasma is suggested, which makes it possible to identify the potentiality of the target plasma hemostasis proenzymes activation.
Highlights
One of the main challenges of modern medicine is the diagnosis and treatment of thrombotic complications
Extracellular proteolytic enzymes of micromycetes from different ecological groups have the ability to act on hemostasis system proteins as activators or direct fibrinolytic agents [4,6]
Twenty micromycete cultures of Aspergillus, Cladosporium, Paecilomyces, Purpureocillum, and Tolypocladium genera were tested for the capacity to produce proteases affecting human hemostatic proteins
Summary
One of the main challenges of modern medicine is the diagnosis and treatment of thrombotic complications. Various thrombolytic agents, which are used in therapy, are proteolytic enzymes with the activity similar to the one of hemostasis system proteases [1]. Such activity was found in proteases present in venom of snakes and in cultures of microorganisms. The preparations obtained on their basis are used as a part of diagnostic kits and as therapeutic agents for the detection and treatment of such complications [2,3,4,5]. Extracellular proteolytic enzymes of micromycetes from different ecological groups have the ability to act on hemostasis system proteins as activators (triggering procoagulant and anticoagulant processes) or direct fibrinolytic agents (breaking down already formed thrombi) [4,6]. There are no unified ways to determine their ability to produce such proteases
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