Abstract
With increased global warming, the impact of high temperature and humidity (HTH) on human health is increasing. Traditional Chinese medicine describes the Herb Yinchen as a remedy for reducing heat and eliminating dampness. This study focused on the impact of HTH conditions on mice and the potential protective effect of Herb Yinchen. Five male Balb/c mouse groups included two normal control groups, two HTH-exposed groups, and one Yinchen-treated group. For either three or ten days, normal and HTH-exposed mice were housed under normal or HTH (33 ± 2 °C,85% relative humidity) conditions, respectively. Yinchen-treated mice, housed under HTH conditions, received the Herb Yinchen decoction for three days. Metabolite profiles of plasma and liver samples from each group were analyzed using LC–MS/MS. Fecal DNA was extracted for 16S rDNA analysis to evaluate the intestinal microbiome. Spearman correlation analysis was performed on metabolites, bacteria, and bile acids that differed between the groups. We found that HTH altered the host metabolite profiles and reduced microbial diversity, causing intestinal microbiome imbalance. Interestingly, Herb Yinchen treatment improved HTH-mediated changes of the metabolite profiles and the intestinal microbiome, restoring them to values observed in normal controls. In conclusion, our study reveals that HTH causes intestinal bacterial disturbances and metabolic disorders in normal mice, while Herb Yinchen could afford protection against such changes.
Highlights
Since the effects of global warming are becoming more serious, research efforts in assessing their impact on public health are substantially increasing worldwide
To study the metabolic changes caused by exposure of mice to the high temperature and humidity (HTH) environment, we investigated the status of plasma metabolites in male Balb/c mice maintained under different conditions
Three and ten days after initiating the experiment, we performed LC–MS/MS analysis to compare the plasma metabolite profiles of mice kept under normal control conditions with those exposed to the HTH conditions
Summary
Since the effects of global warming are becoming more serious, research efforts in assessing their impact on public health are substantially increasing worldwide. Epidemiological studies have shown that global warming has caused an increase in heat-related mortality in many c ountries[1]. Some studies suggest that long-term interactions between high temperature and humidity (HTH) are causing heat waves[7] that can trigger public health emergencies. It was first recorded in the Shen Nong Ben Cao Jing, a Chinese book on medicinal plants. In CM theory, it has the effect of clearing heat, eliminating dampness, and protecting the liver function to provide relief from jaundice. Studies have shown that the ingredients contained in Herb Yinchen. The results of our comprehensive study revealed the effects of the treatment with Herb Yinchen. The results of our comprehensive study revealed the effects of the treatment with Herb Yinchen. on the metabolism in mice subjected to HTH
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