Combined Impact of No-Till and Cover Crops with or without Short-Term Water Stress as Revealed by Physicochemical and Microbiological Indicators.

Abstract

Simple SummaryFarming systems in which no-till (NT) and cover crops (CC) are preferred as alternatives to conventional practices have the promise of being more resilient and climate smart. Our field study aimed to assess the long-term impact of NT plus CC, with vs. without short-term water stress, on soil microbial biodiversity, enzymatic activities, and the distribution of C and N pools within soil aggregates. We found that the diversity of bacteria and fungi in the soil was positively affected by NT + CC, especially under water stress conditions. Under NT + CC, the presence of important plant growth-promoting rhizobacteria was revealed. Soil enzymatic activity confirmed the depleting impact of conventional tillage. Soil C and N were increased under NT + CC due to their inclusion into large soil aggregates that are beneficial for long-term C and N stabilization in soils. Water stress was found to have detrimental effects on aggregates formation and limited C and N inclusion within aggregates. The microbiological and physicochemical parameters correlation supported the hypothesis that long-term NT + CC is a valuable strategy for sustainable agroecosystems, due to its contribution to soil C and N stabilization while enhancing the biodiversity and enzymes.Combining no-till and cover crops (NT + CC) as an alternative to conventional tillage (CT) is generating interest to build-up farming systems’ resilience while promoting climate change adaptation in agriculture. Our field study aimed to assess the impact of long-term NT + CC management and short-term water stress on soil microbial communities, enzymatic activities, and the distribution of C and N within soil aggregates. High-throughput sequencing (HTS) revealed the positive impact of NT + CC on microbial biodiversity, especially under water stress conditions, with the presence of important rhizobacteria (e.g., Bradyrhizobium spp.). An alteration index based on soil enzymes confirmed soil depletion under CT. C and N pools within aggregates showed an enrichment under NT + CC mostly due to C and N-rich large macroaggregates (LM), accounting for 44% and 33% of the total soil C and N. Within LM, C and N pools were associated to microaggregates within macroaggregates (mM), which are beneficial for long-term C and N stabilization in soils. Water stress had detrimental effects on aggregate formation and limited C and N inclusion within aggregates. The microbiological and physicochemical parameters correlation supported the hypothesis that long-term NT + CC is a promising alternative to CT, due to the contribution to soil C and N stabilization while enhancing the biodiversity and enzymes.