Abstract
Combined high pressure and subzero temperature phase transition was performed to inactivate Escherichia coli ATCC 10536 at 200 MPa. Inactivation was compared among atmospheric pressure inactivation (APFI), pressure assisted freezing inactivation (PAFI), pressure assisted thawing inactivation (PATI), and pressure shift freezing inactivation (PSFI). Phase transition characteristics of microbial suspension were concurrently analyzed to investigate its influence on microbial inactivation. Phase transition temperature of E. coli ATCC 10536 suspension decreased to -23.8°C at 200 MPa, which is observed from the phase transition plateau. Supercooling was observed from nucleation temperature (-25°C) of PAFI. PATI resulted in the most inactivation of 4.37 log reduction, followed by PAFI and PSFI in decreasing order. APFI showed no efficacy of inactivation for E. coli ATCC 10536. Synergistic effect of high pressure, subzero temperature, and phase transition was expected for microbial inactivation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
