Combined extracts of Moringa oleifera, Murraya koeingii leaves, and Curcuma longa rhizome increases energy expenditure and controls obesity in high-fat diet-fed rats

Sreenath Kundimi,Krishanu Sengupta,Trimurtulu Golakoti,Nagendra Rao Kundurthi,Venkata Narasimha Rao Tayi,Krishna Chaitanya Kavungala,Barbara Davis,Swaraj Sinha

doi:10.1186/s12944-020-01376-7

Abstract

BackgroundLI85008F is a proprietary combination of leaf extracts of Moringa oleifera, Murraya koeingii, and extract of Curcuma longa rhizome. This herbal extract combination is an effective weight loss supplement for overweight and obese subjects. The present study aimed to investigate the thermogenic potential of the LI85008F in high-fat diet (HFD)-induced obese Sprague Dawley rats.MethodsSeven rats received a regular diet (RD), and twenty-one rats received a high-fat diet (HFD) for 56 days. On day 28, the HFD-fed rats were randomized into three groups (n = 7). Starting from day 29 through day 56, one HFD-fed group received daily oral gavage of 0.5% Carboxymethylcellulose Sodium (CMC) alone (HFD), and the remaining two groups received 100 and 250 mg/kg LI85008F (LI85008F-100 and LI85008F-250, respectively).Body weight, fat mass, fat cell size, liver weight, liver triglyceride were measured. The energy metabolism parameters were measured using indirect calorimetry. In serum, the metabolic and endocrine markers were analyzed. The adipogenic and thermoregulatory proteins expression in the white adipose tissue (WAT) were analyzed using an immunoblot assay.ResultsSupplementation with both doses of LI85008F significantly increased resting energy expenditure (REE) in the obese rats. The LI85008F-250 rats showed significant up-regulation of uncoupling protein-1 (UCP-1) expression, as compared with the HFD rats. LI85008F significantly reduced body weight gain, fat mass, fat cell size, liver weight, and hepatic triglycerides. Serum triglyceride, total cholesterol, glucose, leptin, and fat cell markers were significantly reduced in LI85008F-supplemented rats compared to the HFD rats.ConclusionThe present data suggest that LI85008F reduces body fat mass and controls body weight gain via increasing energy metabolism in combination with reduced lipogenesis in diet-fed obese rats.

Highlights

LI85008F is a proprietary combination of leaf extracts of Moringa oleifera, Murraya koeingii, and extract of Curcuma longa rhizome
Study material LI85008F is a composition of six parts Moringa oleifera leaf aqueous-ethanol extract, three parts Murraya koenigii leaf aqueous-ethanol extract, and one part Curcuma longa rhizome extract standardized to not less than 95% total curcuminoids
LI85008F mitigates body weight gain in high-fat diet-fed obese rats At the end of the 28-day induction phase, the HFD rats showed a significant gain in BW compared with the regular diet (RD) rats (Fig. 1a)

Summary

Introduction

LI85008F is a proprietary combination of leaf extracts of Moringa oleifera, Murraya koeingii, and extract of Curcuma longa rhizome. This herbal extract combination is an effective weight loss supplement for overweight and obese subjects. Obesity is a state of metabolic disorder resulting in an abnormal or excess storage of fat in the body, and it is a potential risk factor for diabetes, cardiovascular diseases, cancer, and other chronic diseases [1]. A series of clinical studies have established that routine physical exercise, consumption of thermogenic food or dietary supplements provide significant benefit in reducing body fat and improve the body composition in overweight or obese subjects [5,6,7,8]

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