Combined effects of retinol, ascorbic acid and α-tocopherol on diurnal variations in rectal temperature of Black Harco pullets subjected to heat stress.

Abstract

The experiment was performed with the aim of determining the effect of co-administration of antioxidant vitamins, retinol, ascorbic acid and α-tocopherol on rectal temperature (RT) fluctuations in pullets during the hot-dry season in Nigeria. Forty-eight Black Harco pullets, aged 16weeks and weighing 1.5 ± 0.03kg were divided by simple random sampling into two groups, consisting of 28 treated and 20 control Black Harco pullets. The RTs of 28 treated and 20 control Black Harco pullets were measured hourly for 3days, 3days apart, from 06:00 to 19:00h (GMT + 1) with a standard clinical thermometer. The treated pullets were administered individually with the vitamins orally in water, while the control pullets were given only water. The lowest hourly RT of 40.9 ± 0.04°C was obtained in treated pullets at 06:00h, while the highest value of 41.1 ± 0.01°C was recorded from 17:00 to 19:00h (P < 0.001). In control pullets, the RT rose significantly from 41.0 ± 0.03°C at 06:00h to the maximum value of 41.6 ± 0.04°C at 15:00h (P < 0.001). The pullets co-administered with retinol, ascorbic acid and α-tocopherol had consistently lower RT values than those of control pullets, especially during the hot hours of the day, from 13:00 to 17:00h. It is concluded that co-administration of retinol, ascorbic acid and α-tocopherol, by preventing a rise in body temperature, ameliorated heat stress, and may enhance productivity of pullets reared under unfavourable, thermal environment conditions.