Combined effects of perchlorate and hexavalent chromium on the survival, growth and reproduction of Daphnia carinata

Abstract

Perchlorate and hexavalent chromium (Cr(VI)) are common cocontaminants in aquatic environments due to their high water solubility, stability, mobility, and some coapplications. However, few studies have investigated their combined toxicity to organisms. In this work, we studied the acute and chronic toxicities of perchlorate and Cr(VI), alone and in combination, with survival, growth, and reproduction as endpoints using Daphnia carinata as a model organism. For a single contaminant, Cr(VI) was found to be more toxic than perchlorate to D. carinata not only in terms of survival but also in terms of growth and reproduction. In regard to the combined pattern, the interactive effects on survival, growth, and reproduction were mainly additivity, antagonism, and synergism, respectively, suggesting that the interactive response of perchlorate and Cr(VI) is endpoint-specific. Due to significant synergism, over 21 days of observation, the inhibition of 0.1 mg/L perchlorate and 0.2 mg/L Cr(VI) on cumulative offspring per female in the first seven broods reached 63.9 ± 3.6%, suggesting that long-term exposure to perchlorate and Cr(VI) at environmentally relevant concentrations may affect D. carinata reproduction in the natural environment. Our results will be significant for understanding the complicated combined toxicity of perchlorate and Cr to aquatic organisms.