Combined effects of LED lights and chicken manure on Neochloris oleoabundans growth

Meltem Altunoz,Onofrio Pirrotta,Luca Forti,Giulio Allesina,Simone Pedrazzi,Olcay Obali,Paolo Tartarini,Laura Arru

doi:10.1016/j.biortech.2017.04.094

Meltem Altunoz, Onofrio Pirrotta + Show 6 more

Open Access

https://doi.org/10.1016/j.biortech.2017.04.094

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Abstract

In this study a photobioreactor prototype is presented for the culture growth of microalgae model organism Neochloris oleoabundans by using chicken manure waste as feedstock along with the optimum combination of led light wavelengths and light intensity. Particularly interesting results are observed on the strains fed by chicken manure medium under the proper combination of red and blue LED light illumination, the microalgal growth resulted comparable with the strains fed by the costly commercial microalgal growth medium (BG 11 medium). Cell concentration, optical density, growth rate, cell size, total lipid and photosynthetic pigment content have been monitored during a time-course experiment. The data suggest that there are difficulties due to white light diffusion into the dark chicken medium, which leads to a generally lower intensity scattered along all wavelengths; blue or combined red and blue lights resulted in a higher irradiation density, affecting microalgae cell growth.

Highlights

In recent years, the non-fossil energy consumption have received growing attention in many countries, China (Zhang, 2016) and USA (Jonathan et al, 2016)
Microalgal species Neochloris oleoabundans was obtained from the University of Modena and Reggio Emilia, Department of Life Science (Reggio Emilia, IT). 1 ml of 14 days old microalgae cell suspension with the initial cell concentration of 0.64 Â 106 cells/ml was transferred into a 250 ml flask containing 100 ml of growth medium (1% v/v)
The growth conditions of this paper will be addressed with the following acronyms: BG11W (BG11 medium, white light); BG11B (BG11 medium, blue light); BG11BR (BG11 medium, blue and red light); BG11-NW; BG11-NB; BG11-NBR; CMW; CMB; CMBR

Summary

Introduction

The non-fossil energy consumption have received growing attention in many countries, China (Zhang, 2016) and USA (Jonathan et al, 2016). Microalgae can be used for secondary metabolite production: antioxidants such as fucoxanthin (Xia et al, 2013), astaxanthin (Hong et al, 2015) and b-carotene (Varela et al, 2015), and vitamins (Grossman, 2016) and anticancer drugs (Borowitzka, 1995). They can even be considered as feed for aquaculture (Sirakov et al, 2015) or animal feed (Kotrbácek et al, 2015)

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