Combined effects of copper and ultraviolet radiation on a microscopic green alga in natural soft lake waters of varying dissolved organic carbon content

Abstract

Selenastrum capricornutum was grown in two lake waters of differing dissolved organic carbon content (1.8 vs. 9.1 mg DOC l −1) to determine the responses of population dynamics and photosynthesis to Cu, and to assess the modifying effects of varying ultraviolet radiation (UVR) exposure. In the absence of UVR, the mean EC 50 for Cu effect on population growth rate was 2.3–2.6 μg l −1 in the low DOC water and 17.4–26.2 μg l −1 in the high DOC water. The variable chlorophyll a fluorescence ratio, F v/ F m, decreased approximately in parallel with the diminished growth rates. Exposure of the higher DOC lake water to full spectrum artificial radiation caused an increase of Cu 2+ concentration, compared to samples held in darkness or in photosynthetically active radiation (PAR) only. Full spectrum exposures also resulted in a lower (although not significantly so) EC 50 for Cu effect on growth rate, consistent with response to the moderately elevated Cu 2+ concentration. Cu 2+ concentration was unaffected by radiation exposure in the low DOC water, and EC 50s for growth were also unaffected except in the most severe UVR treatment, which was >40% inhibited even in the absence of added Cu. Using F v/ F m as an end-point, there was no evidence of interactions between UVR and Cu under the relatively low PAR exposures used here. Algal growth and photosynthesis was extremely sensitive to Cu in these soft lake waters, with EC 50s close to current water quality standards in the low DOC water.