Combined effect of trehalose and spermidine to alleviate zinc toxicity in Vigna radiata.

Abstract

Zinc toxicity is affecting the growth and yield of major crops plants throughout globe by reducing key metabolic processes. In this backdrop, experiments were conducted to evaluate the influence of exogenous supplementation of trehalose (500 µM Treh) and spermidine (500 µM Spd) in alleviating the damaging effects of zinc toxicity (100 µM ZnSO4) in Vigna radiata. Growth, chlorophyll and photosynthesis were reduced due to Zn toxicity; however, exogenous supplementation of trehalose and spermidine not only increased the parameters but also alleviated the decline to considerable levels. Toxicity of zinc increased H2O2, lipid peroxidation and electrolyte leakage by 100.43%, 84.53% and 134.64%, respectively, and application of trehalose and spermidine a reduction of 29.32%, 39.09% and 44.91%, respectively, over the zinc-treated plants. Application of trehalose and spermidine increased the activity of nitrate reductase and the content of nitrogen concomitant with alleviation of the decline caused due to zinc toxicity. The activity of antioxidant system enzymes superoxide dismutase, catalase and the enzymes of ascorbate-glutathione cycle was significantly enhanced due to trehalose and spermidine application. Proline, glycine betaine and activity of γ-glutamyl kinase increased maximally by 281.84%, 126.21% and 181.08%, respectively, in plants treated with zinc + trehalose + spermidine over control. Significant enhancement in the content of total phenols and flavonoids was observed due to the treatment of trehalose and spermidine individually as well as combinedly. Application of trehalose and spermidine reduced the content of methylglyoxal by up-regulating the activity of glyoxylase cycle enzymes. In addition under zinc toxicity conditions, the content of zinc declined in trehalose- and spermidine-treated plants.