Combined effect of single-walled carbon nanotubes and cadmium on human lung cancer cells.

Abstract

Co-exposure of widely used single-walled carbon nanotubes (SWCNTs) and ubiquitous cadmium (Cd) to humans through ambient air is unavoidable. Studies on joint toxicity of SWCNTs and Cd in human cells are scarce. We aimed to investigate the joint effects of SWCNTs and Cd in human lung epithelial (A549) cells. Results showed that SWCNTs were safe while Cd induce significant toxicity to A549 cells. Remarkably, Cd-induced cell viability reduction, lactate dehydrogenase leakage, cell cycle arrest, dysregulation of apoptotic gene (p53, bax, bcl-2, casp3, and casp9), and mitochondrial membrane potential depletion were significantly mitigated following SWCNTs co-exposure. Cd-induced intracellular level of reactive oxygen species, hydrogen peroxide, and lipid peroxidation were significantly attenuated by SWCNT co-exposure. Moreover, glutathione depletion and lower activity of antioxidant enzymes after Cd exposure were also effectively abrogated by co-exposure of SWCNTs. Inductively coupled plasma-mass spectrometry study indicated that higher adsorption of Cd on SCWNTs might decreased cellular uptake and the toxic potential of Cd in A549 cells. Our work warranted further research to explore the potential mechanism of joint effects of SWCNTs and Cd at in vivo levels.