Abstract
Using seedling explants, an improved regeneration protocol was developed for Bauhinia vahlii. A combination of thidiazuron and kinetin (1.0 μM each) increased the number of shoots significantly (p > 0.05) up to four successive subculture cycles. Over 83% shoots rooted on one-fourth strength Murashige and Skoog (MS) medium supplemented with 1.0 μM α-naphthaleneacetic acid (NAA). Fifty percent of plantlets (15 No.) successfully acclimatized in 90 g (w/v) soilrite + sand + soil (2:1:1) in the shed house. Preconditioning at different sucrose concentrations prior to acclimatization showed no effect on percent survival but improved plant quality.
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