Combined detection of extractable nuclear antigen antibody and humoral immunity for the diagnosis of systemic lupus erythematosus

Abstract

Objective To explore the values of combined detection of extractable nuclear antigen(ENA)and humoral immunity in the diagnosis of systemic lupus erythematosus(SLE). Methods All of 88 patients with SLE treated in our hospital from May 2016 to May 2017 were recruited as the study subjects, in which 36 patients were diagnosed with active SLE and 52 patients with inactive SLE.50 individuals who underwent healthy physical examination in the same period were recruited as the study controls.The index of humoral immunity were determined by turbidimetric method.The antibody of ENA were determined by dot-ELISA. Results The immunoglobulin levels(IgA, IgM and IgG)in patients with active SLE were significantly higher than those in the control group, while the levels of complement(C3 and C4)were significantly lower than those in the control group.The immunoglobulins(IgA and IgG)in patients with inactive SLE were significantly higher than those in the control group, while the levels of IgM, C3, and C4 were not significantly different from those in the control group(P>0.05). The anti ENA positive rate, SLE anti double stranded DNA(double strain DNA, ds-DNA)antibody, anti Sm, anti U1 nuclear ribonuclear protein(nRNP)antibody, anti Sjogren syndrome A(SSA)antibody, and anti Sjogren syndrome B(SSB)in both of the active and inactive SLE patients were significantly higher than those in the control group(P<0.05). Conclusion The combined detection of ENA antibody and humoral immunity for SLE was valuable, which could provide information for the development of SLE.This method may also provide guide for the selection of the overall treatment plan for the patients with SLE. Key words: ENA antibody; Humoral immunity; Systemic lupus erythematosus; Diagnosis