Combined cadmium and fluorine inhibit lettuce growth through reducing root elongation, photosynthesis, and nutrient absorption.

Abstract

Cadmium (Cd) and fluorine (F) often coexist in environment and are toxic to organisms; however, their combined effects on plants are still not well documented. In this study, the co-effects of Cd and F on germination, biomass, photosynthesis, and nutrients uptake of lettuce were carried out in hydroponic culture. The results showed that the seed germination and seedling biomass decreased with an increase in Cd and F supplementation. The root morphology verified these effects as excess combined Cd and F diminished the root tips and surface area of lettuce, while single Cd and F inhibited the growth by decreasing root length and average diameter, respectively. These effects were also consistence with a reduction in photosynthesis which was mainly regulated by reducing the quantum yield of PS II, electron transport activity, stomatal conductance, intercellular CO2 concentration, and transpiration rate in response to the pollutants. Moreover, when lettuce exposed to Cd and F stress, the accumulation of several essential elements in shoot decreased. In a sum, the synergistic negative effects of Cd and F on the seed germination and seedling growth of lettuce were observed, and these might be owed to nutrient absorption and translocation in the plant. These findings aid in understanding the harmful effects and specific mechanisms of action of Cd and F on plants.