Abstract
Developed methods for routine analysis of medicines should be considered in terms of analytical efficiency, economic cost, as well as their environmental impact. Different chromatographic methods for the routine quantitative analysis of hederacoside C in ivy leaf extract and its original dosage forms (capsules and syrup) are developed. The performance of HPLC and UPLC methods should be done using ACE C18 (150 mm × 4.6 mm, 5.0 μm) and ACQUITY UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm) columns, respectively, and both of them require a mixture of water and acetonitrile in the ratio 71/29 as a mobile phase. The HPTLC procedure is carried out using the stationary phase pre-coated silica gel 60 F254 glass sheets and a mixture of anhydrous formic acid/acetone/methanol/ethyl acetate (4:20:20:30 v/v). The most suitable conditions of preparation for each sample are established, for instance, the solid-phase extraction (SPE) for the analysis of syrup is applied. Analytical methods are compered by analytical accuracy, calculation of expenses, and assessment of their influence on ecology. All methods are recognized as accurate, precise, and reliable. However, the assessment of the environmental impact shows that HPTLC is the less green method. On the another hand, it is found to be the cheapest, the costs of performing HPTLC are 2.3 and 1.6 times lower than for HPLC and UPLC, respectively.
Highlights
IntroductionLack of access to affordable drugs continues to represent a major global health issue [1,2]
Lack of access to affordable drugs continues to represent a major global health issue [1,2].Medicines that contain plant extracts are essential as a remedy for the treatment of different human diseases [3]
The aim of this study is to develop and validate different chromatographic methods (HPLC, UPLC, and High-Performance Thin Layer Chromatography (HPTLC)) for the assessment of the hederacoside C amount for the standardization of ivy leaf extracts and their original dosage forms, such as capsules and syrup, and choose among them a less costly, more effective, and green technology
Summary
Lack of access to affordable drugs continues to represent a major global health issue [1,2]. Medicines that contain plant extracts are essential as a remedy for the treatment of different human diseases [3]. Such dosage forms are in high demand because of their wide biological action, higher. Safety in comparison with synthetic drugs, and lesser costs [4,5]. The effectiveness of safetypreparation in comparison with synthetic drugs, lessercases, coststheir [4,5].activity
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