Combined antibacterial chemotherapy odontogenic infection-why Ciprofloxacin with Tinidazole?

Abstract

Aim: substantiation and expansion of the range of application in dentistry of the combined antibacterial chemotherapy ciprofloxacin with tinidazole based on the use of automated cultivation of pathogens and scanning electron microscopy of an experimental biofilm. Materials and methods. In the experimental part of the work, strains were used — clinical isolates of obligate-anaerobic bacteria Prevotella intermedia, Fusobacterium nucleatum, Streptococcus sanguis, as well as methicillin-resistant Staphylococcus aureus strain (MRSA) for control. Sequential colonization of the material (methyl polyacrylate) for the formation of the biofilm was performed by S. sanguis, F. nucleatum, P. intermedia. Then, in parallel, the studied preparations were added in working concentrations: amoxicillin+sodium clavulanate and ciprofloxacin+tinidazole (Tsifran ST). To evaluate the activity of chemotherapeutic agents on planktonic forms of bacteria, a system for automated cultivation on the apparatus “RTS-1” (Latvia) was used. To control the biofilm eradication, scanning electron microscopy after 7 days of cultivation. Results. It was found that the MPC of Tsifran ST with automated cultivation of populations was 12.5 mg/l (for ciprofloxacin), which was not inferior to the similar effect of amoxicillin+clavulanate. Unlike the beta-lactam antibiotic, Tsifran ST was also active against the MRSA strain. According to electron microscopy data of a three-component biofilm (S. sanguis, F. nucleatum, P. intermedia) at a concentration of 12.5 mg/l, complete destruction of the biofilm mantle and partial damage to microbial cells by Tsifran ST was established, while amoxicillin+clavulanate did not have such an effect. Conclusion. Ciprofloxacin+tinidazole has an advantage over the amoxicillin+clavulanate for the eradication of an anaerobic biofilm.