Abstract
Root-knot nematodes are the most economically important group of plant-parasitic nematodes. In the present study, functional validation usingin vitroRNAi was carried out onMeloidogyne incognitawith two FMRFamide-like peptide genes,flp-14andflp-18, and a subventral pharyngeal gland specific gene,16D10. It was found that RNAi silencing of each gene reduced the attraction ofM. incognitaat different time intervals both in combination and individually. Silencing of the genes reduced nematode infection by 23-30% and development as indicated by a reduction in the number of females by 26-62%. Reproduction was decreased by 27-73% and fecundity was decreased by 19-51%.In situhybridisation revealed the expression offlp-18in cells associated with the ventral and retro vesicular ganglia of the central nervous system. qRT-PCR supported the correlation between phenotypic effects of silencing with that of transcript quantification.
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