Abstract
ABSTRACTThe bioactive additive toolbox to functionalize supramolecular elastomeric materials expands rapidly. Here we have set an explorative step toward screening of complex combinatorial functionalization with antifouling and three peptide‐containing additives in a bisurea‐based supramolecular system. Thorough investigation of surface properties of thin films with contact angle measurements, X‐ray photoelectron spectroscopy and atomic force microscopy, was correlated to cell‐adhesion of endothelial and smooth muscle cells to apprehend their respective predictive values for functional biomaterial development. Peptides were presented at the surface alone, and in combinatorial functionalization with the oligo(ethylene glycol)‐based non‐cell adhesive additive. The bisurea‐RGD additive was cell‐adhesive in all conditions, whereas the endothelial cell‐specific bisurea‐REDV showed limited bioactive properties in all chemical nano‐environments. Also, aspecific functionality was observed for a bisurea‐SDF1α peptide. These results emphasize that special care should be taken in changing the chemical nano‐environment with peptide functionalization. © 2019 The Authors. Journal of Polymer Science Part B: Polymer Physics published by Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2019, 57, 1725–1735
Highlights
Biomaterial functionalization with various bioactive cues has been studied for several decades to be able to steer cellular behavior and positively influence the response upon implantation of the biomaterial.[1]
When the bisurea-peptide additives were mixed with the PCL-BU polymer, brighter domains were again observed, but here in a different capacity
For all the surfaces with combinations of the BUpeptides and the BOB additive in the PCL-BU base material, the surface appeared to be saturated with additive
Summary
Biomaterial functionalization with various bioactive cues has been studied for several decades to be able to steer cellular behavior and positively influence the response upon implantation of the biomaterial.[1]. One of the most studied examples of such a peptide is the RGD sequence,[5,6,7,8,9] found in for instance fibronectin and vitronectin Besides peptides such as RGD, which induce cell adhesion in general, peptide sequences have been discovered for which specific cell types have an increased affinity over other cell types, due to increased expression of the binding motif on these cells.[2] The REDV peptide, for which endothelial cell-specificity has been reported,[10,11] attributed to binding to integrin α4β1,11,12 has been extensively applied to enhance endothelialization of biomaterials.[13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39] In another approach, a peptide derived from stromal cell derived factor-1α (SDF1α), a chemoattractant of lymphocytes, monocytes, and progenitors cells, has been applied for improved in situ cellularization.[40]
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