Abstract
Combinatorial chemistry involves the chemical or biological synthesis of diverse variation of the structures of a target molecule and the library is then screened for variants of desirable target properties. The approach has been a focus of research activity in drug discovery and biotechnology. This report is to demonstrate the application of enzyme technology using the concept of combinatorial chemistry as a novel approach for the bioconversion of plant fibers. Wheat insoluble fiber was subjected to combinatorial enzyme digestion to create structural variants of feruloyl oligosaccharides (FOS). Fractionation and screening resulted in the isolation of a fraction of bioactive FOS species showing antimicrobial activity. These results demonstrate the feasibility and usefulness of the combinatorial enzyme technique in the transformation of plant biomass to value-added products.
Highlights
Combinatorial chemistry involves the synthesis of a large array of diverse structural variations of the molecule of interest and the constructed library is screened for target variants exhibiting desirable activities and functions [1] [2] [3]
Wheat insoluble fiber was subjected to combinatorial enzyme digestion to create structural variants of feruloyl oligosaccharides (FOS)
These results demonstrate the feasibility and usefulness of the combinatorial enzyme technique in the transformation of plant biomass to value-added products
Summary
Combinatorial chemistry involves the synthesis of a large array of diverse structural variations of the molecule of interest and the constructed library is screened for target variants exhibiting desirable activities and functions [1] [2] [3]. Plant cell wall polysaccharides, such as xylan, pectin, and xyloglucan, consist of polymeric backbones decorated with various side groups that are cleavage targets of specific enzymes The presence of these side groups as well as their positions, density, and types of linkages further influence the pattern of enzymatic degradation of the main chain polymer and vice versa. The hemicellulose polymer xylan contains a β-1,4-xylosyl main chain decorated with at least four types of side groups viz acetyl groups, phenolic-ferulic acids, glucuronyl residues, and arabinofuranosyl residues [7] The cleavage of these side groups requires acetylxylan esterase, feruloyl esterase, β-glucuronidase, α-L-arabinofuranosidase, respectively. Describes combinatorial enzyme digestion of wheat insoluble arabinoxylan to produce libraries of xylo-oligosaccharides, resulting in isolation of FOS species exhibiting specific functional activities
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