Abstract
Objective To investigate the synergistic anti-glioma effects of HSV-tk/GCV system and Recombinant Human Tumor Necrosis Factor-alpha(rhTNF-α)in vitro.Methods The HSV-tk gene was transferred into C6 cells to obtain C6 tk + cells.The test was divided into four groups:C6 cells group、C6tk/GCV group、C6+rhTNF-α group and C6+tk/GCV+rhTNF-α group.Each group was treated with GCV or/and rhTNF-α.The cells survival rates of every group were determined by MTT technique.Results The tumor cells without any anti-glioma factor treatment were growing better and no change was observed in morphology.After 2-3 days,cell axons disappeared,cell bodies crimpled,nucleoli crinkled and cracked in each treated group.The cells survival rates in C6+tk/GCV+rhTNF-α group were lower than that in C6 tk/GCV group and C6+rhTNF-α group ( P 0.05).Conclusion The effect of combination therapy for glioma with HSV-tk/GCV system and rhTNF-α is better than either that of HSV-tk/GCV or rhTNF-α.
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