Combination of Two Kinds of Medicated Microparticles Based on Hyaluronic Acid or Chitosan for a Wound Healing Spray Patch.

Angela Fabiano,Giovanna Batoni,Chiara Migone,Rossella Di Stefano,Giuseppantonio Maisetta,Ylenia Zambito,Anna Maria Piras,Andrea Mezzetta,Luca Cerri,Semih Esin

doi:10.3390/pharmaceutics13122195

Angela Fabiano, Giovanna Batoni + Show 8 more

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https://doi.org/10.3390/pharmaceutics13122195

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Journal: Pharmaceutics	Publication Date: Dec 18, 2021
Citations: 9	License type: CC BY 4.0

Affiliation: University of Pisa, University of Siena

Abstract

Olive leaves extract (OLE) has been extensively studied as antioxidant and antibiotic and these characteristics make it particularly interesting for use on wounds. For this reason, the aim of this study was to introduce OLE in microparticles (MP) of hyaluronic acid (MPHA-OLE) or chitosan (MPCs-OLE) to obtain a spray patch for the treatment of wounds in anatomical areas that are difficult to protect with traditional patches. The MP were characterized for particle size and ability to protect OLE from degradation, to absorb water from wound exudate, to control OLE release from MP. The MPHA and MPCs medicated or not and mixtures of the two types in different proportions were studied in vitro on fibroblasts by the scratch wound healing assay. The MP size was always less than 5 µm, and therefore, suitable for a spray patch. The MPCs-OLE could slow down the release of OLE therefore only about 60% of the polyphenols contained in it were released after 4 h. Both MPHA and MPCs could accelerate wound healing. A 50% MPHA-OLE-50% MPCs-OLE blend was the most suitable for accelerating wound healing. The MPHA-OLE-MPCs-OLE blends studied in this work were shown to have the characteristics suitable for a spray patch, thus giving a second life to the waste products of olive growers.

Highlights

Olive leaves are considered an agricultural by-product obtained during the harvesting or pruning process of olive fruits as they are burned by farmers resulting in the production of greenhouse gases [1]
The leaves from Olea europaea cultivar Olivastra seggianese were considered. This tree has its origins in the Monte Amiata area, its diffusion is limited to the provinces of Grosseto and Siena
The aim of this study was to introduce Olive leaves extract (OLE) in microparticles (MP) of hyaluronic acid (HA) and in MP of chitosan (Cs) to obtain a spray patch for the treatment of wounds in anatomical areas difficult to protect with traditional patches, and in pets or farm animals

Summary

Introduction

Olive leaves are considered an agricultural by-product obtained during the harvesting or pruning process of olive fruits as they are burned by farmers resulting in the production of greenhouse gases [1]. For this reason, olive leaves conversion into higher value products can represent a sustainable and ecological alternative to leaves disposal. The leaves from Olea europaea cultivar Olivastra seggianese were considered This tree has its origins in the Monte Amiata area (southern Tuscany, Italy), its diffusion is limited to the provinces of Grosseto and Siena. The olive leaf extract (OLE)—as well as olive oil—has been extensively studied for its antimicrobial and antioxidant characteristics, and as an antihypertensive, antiatherogenic, anti-inflammatory, hypoglycemic, or hypocholesterolemic

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