Abstract
In this study, the authors examined combinations of growth factors that induce effective chondrogenesis from adipose tissue-derived mesenchymal stem cells (MSCs). Human MSCs were isolated from bone marrow (BMMSCs) and adipose tissue (ATMSCs) and characterized according to flow cytometry for CD34, CD45, CD73, and CD166. Chondrogenesis was induced by culturing ATMSCs in pellets without growth factors (negative control) and with 5 ng/mL of transforming growth factor beta 2 (TGF-beta(2)), 100 ng/mL of bone morphogenetic protein (BMP)-2, 100 ng/mL of BMP-6, 100 ng/mL of BMP-7, 5 ng/mL of TGF-beta(2) and 100 ng/mL of BMP-2, 5 ng/mL of TGF-beta(2) and 100 ng/mL of BMP-6, and 5 ng/mL of TGF-beta(2) and 100 ng/mL of BMP-7. BMMSCs cultured under the same condition with 5 ng/mL of TGF-beta(2) were used as positive controls. Flow cytometry showed that ATMSCs and BMMSCs had similar surface marker profiles. After 4 weeks of in vitro culture, glycosaminoglycan assays, real-time reverse transcriptase polymerase chain reaction, and histological findings demonstrated that the combination of 5 ng/mL of TGF-beta(2) and 100 ng/mL of BMP-7 most effectively induced chondrogenesis from ATMSCs. The findings of this study suggest that the combination of TGF-beta(2) and BMP-7 potently enhances chondrogenesis from ATMSCs and can be used to overcome the inferior chondrogenic potential of ATMSCs in cartilage tissue engineering.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
