Combination of transcriptome sequencing and iTRAQ proteome reveals the molecular mechanisms determining petal shape in herbaceous peony (Paeonia lactiflora Pall.)

Yanqing Wu,Jiali Shang,Daqiu Zhao,Yuhan Tang,Jun Tao,Yu Jiang

doi:10.1042/bsr20181485

Yanqing Wu, Jiali Shang + Show 4 more

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https://doi.org/10.1042/bsr20181485

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Abstract

The molecular mechanisms controlling petal shape in a herbaceous peony, Paeonia lactiflora Pall., a popular high-grade cut flower worldwide, remain unclear. Here, we selected inner and outer petals from P. lactiflora ‘ZiFengyu’ with an anemone type as the study object. Using transcriptome sequencing and isobaric tags for relative and absolute quantitation proteome, 979 differentially expressed genes and 266 differentially expressed proteins were detected within the inner and outer petals. Of these, the present study identified a key gene APETALA2 that regulates flower shape development. In addition, we obtained a 1935 bp full-length cDNA sequence of APETALA2 by rapid amplification of cDNA ends amplification. Through further validation using quantitative real-time polymerase chain reaction and Western blot analysis, APETALA2 showed a markedly higher expression in outer than that in inner petals. Therefore, the present study indicates that the increased expression of APETALA2 contributes to the formation of petals in P. lactiflora.

Highlights

Paeonia lactiflora Pall., a flowering plant, is native to China
Zhao et al [1] have systematically analyzed the molecular mechanism of color formation in P. lactiflora with red outer petals and yellow inner petals using RNA sequencing technology, and identified potential candidate genes that could be involved in the flavonoid metabolic pathway including phenylalanine ammonia-lyase (PAL), flavanone-3-hydroxylase (F3H), flavonol synthase (FLS), and anthocyanidin synthase (ANS)
The molecular mechanisms underlying the regulation of flower shape have been mainly studied using model plants including Arabidopsis and petunia [2,3,4,5,6], suggesting the molecular models known as ABCDE models regulate flower development

Summary

Introduction

Paeonia lactiflora Pall., a flowering plant, is native to China. This traditional floral symbol of China is renowned as the king of flowers together with other peony species. Flower color is an important determinant for ornamental value, and the flower organ is an ideal model system for studying the relationship between plant development, genetics, and evolution. The flower color and organ development of P. lactiflora have become important research topics. The molecular mechanism involved in the floral formation has been well studied in P. lactiflora. The petal pattern or flower shape is one of the important indicators for ornamental merit; an increasing number of studies have focused on the petal formation pattern of ornamental plants. The molecular mechanisms underlying the regulation of flower shape have been mainly studied using model plants including Arabidopsis and petunia [2,3,4,5,6], suggesting the molecular models known as ABCDE models regulate flower development.

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