Abstract
Objective: The present study was undertaken as a baseline study to evaluate the nuclear diameter (ND), cell diameter (CD) and nuclear-cytoplasmic ratio (N: C) and their variation with age in normal buccal smears of female subjects. Study Design: Buccal smears were collected from 400 apparently healthy individuals and were stained using the standard Papanicolaou laboratory procedure. The CD and ND were measured using a stage micrometer and an ocular micrometer. Results: The results showed an increase in ND and CD from the 5-10 age groups to 15-35 age groups. In the 15-35 age groups, there was a decline in ND and CD when comparing the first and the second half of the menstrual cycle. There was further decline in ND and CD in the 45-60 age groups. Conclusions: Age-related alterations with regard to size and pattern of maturation of cells were observed. This study proves that ageing has profound impact on the buccal mucosal cell size.
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